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Supplemental data

Table 1. Sequences (listed 5' to 3') of crRNAs used for DNAH5 and CCDC40 experiments. DNAH5 KO experiment performed with single guide, CCDC40 KO experiment performed with two guide approach.

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Table 2. Sequences (listed 5' to 3') of PCR amplification and sequencing primers for the locus listed.

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Table 3. Efficiency of gene editing for each gene listed. After isolation and passaging, clones were screened for monoallelic or biallelic deletions of the targeted regions.

Berical A, Kiyokawa H, et al. Airway disease modeling with gene-edited human basal cell transplantation. AJRCCM 2024.

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